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rabbit polyclonal ampk alpha antibody  (Proteintech)


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    Proteintech rabbit polyclonal ampk alpha antibody
    Rabbit Polyclonal Ampk Alpha Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+ampk+alpha+antibody/AMPK+alpha+1+Antibody/pm41380676-429-24-39
    Average 96 stars, based on 241 article reviews
    rabbit polyclonal ampk alpha antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Macrophage PD-1 regulates energy expenditure and metabolic dysfunction under immune checkpoint blockade.
    Article Snippet: In brief Wu et al. identified a moonlighting function of macrophage PD-1 in protecting against high-fat diet-induced obesity and the obesity-associated inflammatory microenvironment by suppressing ER stress-mediated systemic inflammatory responses.. Their findings highlight the critical role of macrophage PD-1 at the intersection of immune checkpoint blockade, energy expenditure, and metabolic dysfunction.



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    rabbit polyclonal ampk alpha antibody  (Proteintech)
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    Proteintech rabbit polyclonal ampk alpha antibody
    Rabbit Polyclonal Ampk Alpha Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+ampk+alpha+antibody/AMPK+alpha+1+Antibody/pm41380676-429-24-39
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    ampk alpha  (OriGene)
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
    Rabbit Polyclonal Anti Ampk α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti ampk alpha  (Proteintech)
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
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    rabbit anti ampkα2  (Bioss)
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    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
    Rabbit Anti Ampkα2, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+ampk+alpha+antibody/AMPK+alpha+2+Polyclonal+Antibody/pm40334811-88-133-135
    Average 92 stars, based on 1 article reviews
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    rabbit anti ampk  (Bioss)
    94
    Bioss rabbit anti ampk
    <t>AMPK/mTOR</t> signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.
    Rabbit Anti Ampk, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+ampk+alpha+antibody/AMPK+alpha-1+Polyclonal+Antibody/pm40065050-75-32-34
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    Image Search Results


    AMPK/mTOR signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.

    Journal: The Journal of Biological Chemistry

    Article Title: Metformin attenuates O-GlcNAc modification to improve renal function via AMPK/mTOR signaling in diabetic nephropathy

    doi: 10.1016/j.jbc.2025.110909

    Figure Lengend Snippet: AMPK/mTOR signaling pathway influences mesangial cell damage via O-GlcNAcylation under high-glucose treatment. A , representative Western blot images of four groups of O-GlcNAc, OGT, and OGA and AMPK/mTOR pathway proteins according to whether or not AMPK agonist and inhibitor were added, n = 3. B , representative quantification of O-GlcNAc, OGT, and OGA in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. C , mRNA expression of OGT and OGA assessed by RT-PCR, n = 3. D–F , representative quantification of p-AMPK, p-mTOR, and p-S6K in MCs of four groups according to whether or not an AMPK agonist and inhibitor was added, n = 3. G , the effect of AMPK agonist and inhibitor treatment on MCs volume under high glucose conditions, n = 3. H , the effect of AMPK agonist and inhibitor treatment on MCs viability under high-glucose conditions, n = 3. Data are presented as mean ± SD. Significance analysis was performed with one-way ANOVA (multiple comparisons). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. AMPK, AMP-activated protein kinase; CC, compound C; MC, mesangial cell; Met, Metformin; mTOR, mammalian target of rapamycin; OGA, O-GlcNAcase; OGT, O-GlcNAc transferase.

    Article Snippet: The following primary antibodies were used to detect the respective proteins after incubation overnight at 4 °C in 5% BSA: O-GlcNAc (#65292-1-lg, proteintech, 1:1000), OGT (# R25212 , Zen Bio, 1:1000), OGA (# R24971 , Zen Bio, 1:1000), Phospho-AKT (Ser473) (#310021, Zen Bio, 1:1000), AKT (# R23412 , Zen Bio, 1:1000), Phospho-mTOR (Ser2448) (#381557, Zen Bio, 1:1000), mTOR (#380411, Zen Bio, 1:1000), Phospho-AMPK alpha 1/2 (Thr183/Tyr172) (#340763, ZenBio, 1:1000), AMPK alpha (#23314, ZenBio, 1:1000), Phospho-P70 S6K (Thr389) (#AF5899, Beyotime, 1:1000), P70 S6K (#AF0258, Beyotime, 1:1000), and GAPDH (#TA802519, Origene, 1:1000).

    Techniques: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction